Figure 2. Target specificity and retrograde labeling efficacy of envA-pseudotyped RVdG-CVS-N2c particles, produced in BHK-eT cells.
(A) Illustration of the injection scheme (left) and corresponding representative confocal images demonstrating the targeting specificity of CVS-N2c particles (green) to FLAG3X tagged cells (magenta) along with retrograde labeling to EC layer 2 cells (rightmost image, EC layers are separated by dashed lines and their numbers are denoted above). (B) Illustration of the injection scheme (top left) and corresponding representative confocal images demonstrating efficiency and specificity of CVS-N2c-mediated retrograde labeling from adult-born DGCs. (C) Illustration of the injection scheme (top left) and corresponding representative confocal images demonstrating target specificity, with no accompanying retrograde labeling, in the absence of the rabies glycoprotein in any of its projections in four separate experiments.
Figure 2—figure supplement 1. Transsynaptic retrograde labeling pattern from adult-born hippocampal DGCs in subcortical regions.
