Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (mouse Prox1cre) | Tg(Prox1-cre)SJ32Gsat/Mmucd | MMRRC (N. Heintz) | 036644-UCD | |
Strain, strain background (mouse Calb1cre) | B6;129S-Calb1tm2.1(cre)Hze/J | Jackson labs (H. Zeng) | 028532 | |
Strain, strain background (mouse Ascl1creERT2) | Ascl1tm1.1(Cre/ERT2)Jejo/J | Jackson labs (J. Johnson) | 012882 | |
Strain, strain background (mouse Ai14) | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Jackson labs (H. Zeng) | 007914 | |
Strain, strain background (mouse GAD1EGFP) | Not deposited | K.Obata and Y.Yanagawa Tamamaki, N., Yanagawa, Y., Tomioka, R., Miyazaki, J.I., Obata, K., and Tamamaki et al., 2003. Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse. Journal of Comparative Neurology 467, 60–79. https://doi.org/10.1002/cne.10905. |
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Strain, strain background (mouse Dlx5/6FlpE) | Tg(mI56i-flpe)39Fsh/J | Jackson labs (G. Fishell) | 010815 | |
Strain, strain background (mouse RCE-FRT) | Gt(ROSA)26Sortm1.2(CAG-EGFP)Fsh/Mmjax | Jackson labs (G. Fishell) | 32038 | |
Strain, strain background (mouse Ai65) | B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J | Jackson labs (H. Zeng) | 010815 | |
Cell line (human) | HEK293T | ATCC | CRL-3216 | |
Cell line (hamster) | BHK-21 | ATCC | CCL-10 | |
Cell line (human) | HEK-GT | This paper | ||
Cell line (human) | HEK-TVA | This paper | ||
Cell line (hamster) | BHK-eT | This paper | ||
Recombinant DNA reagent (plasmid) | pCAG-B19N | AddGene (I. Wickersham) | #59924 | |
Recombinant DNA reagent (plasmid) | pCAG-B19P | AddGene (I. Wickersham) | #59925 | |
Recombinant DNA reagent (plasmid) | pCAG-B19L | AddGene (I. Wickersham) | #59922 | |
Recombinant DNA reagent (plasmid) | pAdDeltaF6 | AddGene (J. Wilson) | #112867 | |
Recombinant DNA reagent (plasmid) | rAAV-DJ RepCap | Mark A. Kay | ||
Recombinant DNA reagent (plasmid) | rAAV2-retro helper | AddGene (A. Karpova and D. Schaffer) | #81070 | |
Recombinant DNA reagent (plasmid) | pAAV-EF1a-Cre | AddGene (K. Deisseroth) | #55636 | |
Recombinant DNA reagent (plasmid) | pAAV-DIO-hSyn-mCherry | AddGene (K. Deisseroth) | #114472 | |
Recombinant DNA reagent (plasmid) | RVdG-RVDG-CVS-N2c-EGFP | AddGene (T. Jessell) | #73461 | |
Recombinant DNA reagent (plasmid) | RVdG-RVDG-CVS-N2c-tdTomato | AddGene (T. Jessell) | #73462 | |
Recombinant DNA reagent (plasmid) | pAAV-DIO-Ef1a-TVA-2A-oG | This paper | #172359 | |
Recombinant DNA reagent (plasmid) | pAAV-DIO-Ef1a-TVA-2A-N2cG | This paper | #172360 | |
Recombinant DNA reagent (plasmid) | pAAV-FRT-EF1a-TVA-2A-N2cG | This paper | #172361 | |
Recombinant DNA reagent (plasmid) | pAAV-DIO-CaMKII-TVA-P2A-N2cG | This paper | #172362 | |
Recombinant DNA reagent (plasmid) | pAAV-SEO-CaMKII-TVA-P2A-N2cG | This paper | #172363 | |
Recombinant DNA reagent (plasmid) | pAAV-mDLX-TVA-2A-N2cG | This paper | #172364 | |
Recombinant DNA reagent (plasmid) | pAAV-DIO-mDLX-TVA-2A-N2cG | This paper | #172365 | |
Recombinant DNA reagent (plasmid) | pAAV-DIO-CAG-TVA-P2A-dTomato | This paper | #177016 | |
Recombinant DNA reagent (plasmid) | pAAV-DIO-EF1a-TVA-P2A-EYFP | This paper | #177017 | |
Recombinant DNA reagent (plasmid) | pAAV-SEO-CaMKII-EGFP | This paper | #177018 | |
Recombinant DNA reagent (plasmid) | MMLV-CAG-TVA-IRES-Puro | This paper | #172366 | |
Recombinant DNA reagent (plasmid) | MMLV-CAG-SADB19_oG-IRES-Puro | This paper | #172367 | |
Recombinant DNA reagent (plasmid) | MMLV-CAG-G_oT7pol-IRES-BSD | This paper | #172369 | |
Recombinant DNA reagent (plasmid) | pLV-EF1a-N2c_envA-IRES-Neo | This paper | #172368 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-tdTomato-ChIEF | This paper | #172370 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-EGFP-ChIEF | This paper | #172371 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-EGFP-iCre | This paper | #172372 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-EGFP-FlpO | This paper | #172373 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-tdTomato-iCre | This paper | #172374 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-tdTomato-FlpO | This paper | #172375 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-mTurquoise | This paper | #172376 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-E2_Crimson | This paper | #172377 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-nl.mCherry-FlpO | This paper | #172378 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-nl.EGFP-FlpO | This paper | #172379 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-nl.EGFP-SypGFP | This paper | #172380 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-SypRFP | This paper | #172381 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-nl.EGFP-tdTomato | This paper | #172382 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-EYFP | This paper | #172383 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-mCitrine | This paper | #172384 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-nl.mCherry-GCaMP7s | This paper | #172385 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-nl.EGFP-jRGECO1a | This paper | #172386 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-GCaMP8f | This paper | #172387 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-GCaMP8m | This paper | #172388 | |
Recombinant DNA reagent (plasmid) | RVDG-CVS-N2c-GCaMP8s | This paper | #172389 | |
Antibody | Anti parvalbumin (rabbit polyclonal) | Swant antibodies | PV-27 | 1:1000 dilution |
Antibody | Anti somatostatin (rabbit polyclonal) | BMA Biomedicals | T-4102 | 1:1000 dilution |
Antibody | Anti EGFP (Chicken polyclonal) | Abcam | AB13970 | 1:1000 dilution |
Antibody | Anti FLAG (mouse monoclonal) | Sigma Alderich | F1804 | 1:1000 dilution |
Antibody | Alexa Fluor 647-conjugated goat anti-rabbit | Invitrogen | A-21244 | 1:1000 dilution |
Antibody | Alexa Fluor 647-conjugated goat anti-mouse | Invitrogen | A-21235 | 1:1000 dilution |
Antibody | Alexa Fluor 488-conjugated goat anti-chicken | Invitrogen | A-11039 | 1:1000 dilution |