Table 2. Pseudotyping of rescued vectors.
| BHK-EnvA (Osakada and Callaway, 2013) | Neuro2a-envA (Reardon et al., 2016) | BHK-eT | |
|---|---|---|---|
| Stably-expressed transgenes | envA or envB | envA | envA +TVA |
| Selection markers | Fluorescence | Fluorescence | Antibiotic resistance genes |
| Growth conditions | 3% CO2 at 35 °C | 3% CO2 at 35 °C | 5% CO2 at 37 °C |
| Pseudotyping timeline | 7–10 days | 28 days | 4–6 days |
| Requirements for pseudotyping | Large stock of native-coat particles | Large stock of native-coat particles | Trace amounts of either native-coat or evA pseudotyped stock |
| Titer | Low 10^8 typical | Low 10^7 typical | High 10^9 typical |
| Native-coat background | 10^2 typical | Not detectable | Not detectable |