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. Author manuscript; available in PMC: 2022 Sep 15.
Published in final edited form as: Nat Struct Mol Biol. 2021 Dec 9;28(12):1029–1037. doi: 10.1038/s41594-021-00690-7

Fig. 2. Get4 primes Get3 for TA protein loading.

Fig. 2.

a, Clipped view of the sharpened cBUGG-in map (at 9.5σ contour level) showing the interior of the Get3 substrate chamber. Get3 helices (numbered) lining the chamber and putative Get3 (green) and Get4 (yellow) lid (L) helices are indicated. Green arrows, Get3 L(α4) interaction with Get4. b, Get3 model showing how L(α4) connects helices (α5, α6) lining the substrate chamber with β3 in the ATPase domain (green). The Switch I loop is purple; the other Get3 subunit is light gray. c, Segmented cBUGG-in map and model of Get3 L(α4). d, The Get3 L(α4) (green) and Get4 (light orange) interaction site. e, Surface electrostatics of the regions boxed in panel d.