TABLE 3.
TnphoA and Tn10dCm swarming mutantsa
| Type of function affected and mutantb | Swarming phenotype on:
|
P22 resistancec | Mutant gened | Function | Reference | |
|---|---|---|---|---|---|---|
| Difco medium | Eiken medium | |||||
| Metabolism | ||||||
| 8-11* | − | + | s | hisD | Histidine biosynthesis | 19 |
| 17-7* | − | + | s | hisD | Histidine biosynthesis | 19 |
| 64-7* | − | + | s | hisD | Histidine biosynthesis | 19 |
| 16-48* | − | + | s | cybC | Cytochrome synthesis | 96 |
| L6-393 | − | + | s | dhnA | Carbohydrate metabolism | 94 |
| S28-14 | − | + | s | purH | Purine biosynthesis | 20 |
| S122-27 | − | + | s | iadA | Peptide metabolism | 30 |
| M151-44 | − | + | s | fadH | Fatty acid metabolism | 106 |
| M176-16 | − | + | s | fdhF | Formate metabolism | 104 |
| Surface or membrane | ||||||
| 16-6* | − | + | s | wcaK | Capsular polysaccharide region | 87 |
| AF20* | − | + | r | waaKe | LPS biosynthesis | 73 |
| 58-19* | − | + | r | waaPe | LPS biosynthesis | 73 |
| L9-50 | − | + | s | shdA | Similar to adhesin | GenBank accession no. AF140550 |
| S82-3 | − | + | s | ompS1 | Outer membrane protein | 25 |
| S126-45 | − | + | s | sfiX | Cell wall biosynthesis | 27 |
| M17-48 | − | + | s | marA | Membrane permeability | 90 |
| M210-43 | − | + | s | corA | Magnesium transport | 43 |
| Signaling | ||||||
| L5-192 | − | + | s | baeS | Sensor kinase | 68 |
| M208-35 | − | + | s | yfhK | Putative sensor kinase | 13 |
| S28-31 | − | + | s | yojN | Putative sensor kinase | 13 |
| Other | ||||||
| L5-225 | − | + | s | xerC | Chromosome segregation | 12 |
| M112-47 | − | + | s | ydhC | Unknown | 21 |
| M153-43 | − | + | s | yihP | Unknown | 13 |
| M156-250 | − | + | s | ybeS | Unknown | 13 |
| M17-39 | − | + | s | yehE | Unknown | 13 |
| L7-85 | − | + | s | UN-1 | ||
| S71-32 | − | + | s | UN-1 | ||
| S80-24 | − | + | s | UN-2 | ||
| S116-25 | − | + | s | UN-2 | ||
| S118-35 | − | + | s | UN-1 | ||
| M16-43 | − | + | s | UN-1 | ||
| M156-29 | − | + | s | UN-2 | ||
| M209-40 | − | + | s | UN-1 | ||
a
A total of 35,000 mutants (10,000 TnphoA and 25,000 Tn10dCm) were screened. There were 311 swarm-negative mutants (235 LPS, 44 Che, and 32 others), and 34 were sequenced.
b
Asterisks indicate TnphoA insertions; all others have Tn10dCm insertions.
c
r, resistant; s, sensitive
d
UN-1 and UN-2, contiguous sequences (contigs) identified in the S. enterica serovar Typhimurium (Washington University) and S. typhi (BLAST) unfinished genome databases, respectively.
e
LPS core modification.