Figure 4.

SARS-CoV-2 infected HLOs promote macrophage expression of MCP-1 in an IL-6-dependent manner

(A) Experimental schematic of co-culture of SARS-CoV-2 infected HLOs with hPSC-derived macrophages (HMacs). Infected HLOs were incubated with HMacs beginning at 48 h post-infection in the presence or absence of Tocilizumab (5 μg/mL).

(B) Gene set enrichment analysis (GSEA) showing the top upregulated and gene sets in infected HLO cultured with HMacs versus infected HLOs not cultured with HMacs.

(C) RT-qPCR of SARS-CoV-2 nucleocapsid (NC) expression in HLOs and HMacs 96 h after the initiation of co-culture.

(D) RT-qPCR of Monocyte chemoattractant protein-1 (MCP-1) expression in HMacs 96 h after the initiation of co-culture with infected HLOs. All samples were plated in triplicate for RT-qPCR.