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. 2022 Sep 1;8(9):1038–1051. doi: 10.1038/s41477-022-01226-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — The metabolites identified in all 6 biological replications were used in this study. A, Overview of the metabolomics dataset of Ceratopteris based on HMDB (Human Metabolome Database, https://hmdb.ca). All compounds were divided into 11 clades according to the HMDB superclass. The compound numbers in each superclass were separated as positive and negative metabolites. B, KEGG enrichment of putative compounds related to human diseases. C, The Flavonoid Biosynthesis pathway in Ceratopteris. 4CL, 4-coumarate:CoA ligase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3-hydroxylase; F3’H, flavonoid 3’-hydroxylase; FLS, flavonol synthase; OMT1, O-methyltransferase; UGT, UDPdependent glycosyltransferase; DFR, dihydroflavonol 4-reductase; LDOX/ANS, leucoanthocyanidin dioxygenase/anthocyanidin synthase; AAT, anthocyanin acyltransferase.

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