Extended Data Fig. 9. Activity assays support PIN8 is independent of ions, pH and lipids.
A) Peak currents elicited by 100 µM IAA in Na+-free K+ buffer or K+-free Na+ buffer. The current response was independent of the principal cation. Bars are mean ± SE; n = 3. The points represent individual measurements. Means were compared by a two-tailed unpaired t-test (p = 0.62). B) Peak currents elicited by 100 µM IAA, and with proton-motive force decouplers CCCP and DNP present. The current responses were similar in all cases. Bars are mean ± SE; n = 3. The points represent individual measurements. No difference (p = 0.97) between groups was found by one-way ANOVA multiple comparisons, followed by Tukey’s Post Hoc test (IAA vs. IAA+CCCP p > 0.99, IAA vs. IAA+DNP p = 0.98, IAA+CCCP vs. IAA+DNP p = 0.98). C) Oocyte export assay using 3H-IAA and PIN1 plus kinase PID as a control. PIN8 transport rate is unchanged at two different external pH values; n = 2. Data points are biologically independent experiments. The mean is indicated