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. 2022 Aug 17;609(7927):597–604. doi: 10.1038/s41586-022-05084-3

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — A, Top, three-colour imaging of eGFP::WSP1; ARX2::tagRFP and LifeAct::HaloTag; zoomed insets and bottom show a representative cortical condensate example (boxed) together with its time evolution, revealing that the order of arrival of components is WSP-1 followed by ARX-2 and F-actin. B, Top, two-color imaging of CAP-1::eGFP; Life-Act::mkate2, zoomed insets and bottom show a representative cortical condensate example (boxed) together with its time evolution, revealing that that CAP-1 comes after F-actin. C, Comparing the temporal order of components in A, B by alignment in time with respect to the maximum LifeAct signal reveals that the order of components recruitment is WSP-1, ARP2/3, F-actin, and CAP-1. D, Top, two-color imaging of CAP-1::eGFP; LifeAct::mkate2, zoomed insets and bottom show a representative cortical condensate example (boxed) together with its time evolution. CYK-1/Formin is not present in cortical condensates, but found in contractile actomyosin pulses just prior to large-scale contractions. E, Labeling F-actin via Utrophin (left) leads to similar time series as when labeling F-actin via LifeAct (right). Bottom images, respective kymographs determined along dotted white line. Note that in the condition where both LifeAct and Utrophin were imaged (right) colocalization is high between the two channels in the first couple of frames only due to differential bleaching. Scale bars, 10µm.