Extended Data Fig. 5. Volume-independent stoichiometry evolution sets internal concentrations also for moderate arx-2 RNAi.
A, Volume vs. F-actin volume fraction phase portrait obtained by a change of variables of the moderate arx-2 RNAi data represented in Fig. 3. Orange-grey arrows, measured as in Fig. 2c. Yellow-green-blue, calculated using the empirically determined growth laws. Colors denote time rate change vector magnitudes. The time evolution of effective F-actin volume fraction is independent of volume (also see supplement). B, Rate of change of volume (blue-red heatmap) as a function of instantaneous volume and effective F-actin volume fraction. Condensates transition from growth to shrinkage at an effective F-actin volume fraction of 0.5 (orange-dashed line), which corresponds to the region of slow kinetics within the transition region (Fig. 2d, Fig. 4c,d). C, Mass flux phase portrait current magnitude. Contour lines (68%, 25%) depict the most commonly occupied total intensity values. The orange dashed line indicates the effective F-actin volume fraction corresponding to the center of the transition region (Fig. 2d), and coincides with lowest currents and slowest kinetics. D, Concentration flux phase portrait current magnitude. Contour lines (68%, 25%) depict the most commonly occupied concentration values and reflect the preferential maintenance of a pair of concentrations. This pair of concentrations lies at the intersect of the line of constant total density (yellow) and the line of dominant stoichiometry (orange) which corresponds to the orange lines in (A-C) and to the transition regions of Fig. 2d.