Extended Data Fig. 7. Ccr9−/− T cells home into the intestinal tissue after adoptive transfer.
a, Representative flow cytometry analysis of intestinal T cells in Rag2−/− mice two weeks post-adoptive transfer of either Ccr9+/+ or Ccr9−/− T cells from one of two independent experiments. b, Absolute numbers of total TCRβ+ T cells, as well as CD8α+ and CD4+ T cells subsets, in the small intestine of Rag2−/− mice two weeks post-adoptive transfer of either control Ccr9+/+ (n = 3) or Ccr9−/− (n = 3) T cells. c, Immunofluorescence of ileum from Rag2−/− mice adoptively transferred with Ccr9−/− T cells (scale bar 50 µm). Data are representative of two independent experiments. d-e, Representative flow cytometry analysis of intestinal CD8a+ (d) and CD4+ (e) T cell subsets in Rag2−/− mice two weeks post adoptive transfer of Ccr9−/− T cells from one of two independent experiments. Frequencies of CD4+ T cells in the small intestine of Rag2−/− mice two weeks post-adoptive transfer of either control Ccr9+/+ (n = 4) or Ccr9−/− (n = 6) T cells. Results in were obtained in three independent experiments. Each bar corresponds to the mean ± s.e.m of the values obtained (ns, not significant; *P < 0.05; two-tailed Mann-Whitney test; exact P values are provided in the source data).