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. 2022 Aug 23;23(9):1317–1323. doi: 10.1038/s41590-022-01284-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Representative flow cytometry analysis for the identification of Ncr1^GFP+Il22^TdT+ ILC3s in intestinal CD45⁺ cells from Ncr1^GFPIl22^TdT reporter mice. Data are representative of three independent experiments. b, Time-lapse images (scale bar, 15 µm) of Ncr1^GFP+Il22^TdT+ cells in intestinal villi. Mean speed and arrest coefficient of Ncr1^GFP+Il22^TdT+ ILC3s (n = 36) compared to Ncr1^GFP−Il22^TdT+ cells (n = 95) c, Representative flow cytometry analysis of intestinal Ncr1^GFP+Il22^TdT+ ILC3s, pregated on CD45⁺ cells from one of two independent experiments and absolute numbers of Ncr1^GFP+Il22^TdT+ ILC3s (n = 4 mice per condition) in Ncr1^GFPIl22^TdT mice treated or not with flagellin 5 h before. d, Time-lapse images (scale bar, 15 µm) of Ncr1^GFP+Il22^TdT+ ILC3s in intestinal villi 5 h post-flagellin stimulation as in c. Mean speed distribution of intestinal Ncr1^GFP+Il22^TdT+ ILC3s, with or without flagellin. e–h, Individual tracks (e), mean speed and arrest coefficient (f), distribution of ILC3 patrolling behaviors (g) and MSD (h) of Ncr1^GFP+Il22^TdT+ ILC3s as in c (n = 29 and 156). Results are from at least nine movies per condition obtained in three independent experiments. Each bar corresponds to the mean ± s.e.m. of the values obtained; only Ncr1^GFP+Il22^TdT+ ILC3s were tested (*P < 0.05; ***P < 0.001; two-tailed Mann–Whitney U-test; exact P values are provided in the source data).

Source data