Extended Data Fig. 1. BM chimera, a novel model to investigate ILC3 dynamics in vivo.

a, Representative gating strategy for identification of ILC3s from small intestine lamina propria of C57BL6 and Rorc^GFPIl22^TdT mice from one of three independent experiments (ILC3, CD45⁺ CD3⁻ CD19⁻ CD90⁺ CD127⁺ NK1.1⁻ RORγt⁺ cells; NK cells, CD45⁺ CD3⁻ CD19⁻ NKp46⁺ NK1.1⁺ RORγt⁻ cells). b, Representative flow cytometry analysis of small intestine from Rorc^GFPIl22^TdT from one of three independent experiments. Representative intravital image of ILC3s (scale bar, 50 µm) in intestinal villi (V) of Rorc^GFP Il22^TdT mice. Mice were injected with Hoechst and Evans blue before imaging to visualize all nuclei (blue) and blood vessels (magenta). Data are representative of three independent experiments. c, Representative intravital image (scale bar 50 µm) and flow cytometry analysis of small intestine from Rag2^−/−Rorc^GFPIl22^TdT reporter mice from one of three independent experiments. c, Bone marrow (BM) chimeric mice were created by cell transfer. Mixed bone marrow cells from Rag2^−/−Rorc^GFPIl22^TdT (95%) and Actb^ECFP (5%) mice was injected into lethally irradiated congenic C57BL/6 J mice and later analyzed for reconstitution. Representative flow cytometry analysis of reconstitution by donor cells (CD45.2⁺) in the intestinal lamina propria from one of two independent experiments.