Extended Data Fig. 2. NKp46⁺ ILC3 responses upon bacterial challenge.

a, b, Representative flow cytometry analysis of small intestine leukocytes from Ncr1^GFPIl22^TdT mice at indicated conditions from one of three independent experiments. c, Representative flow cytometry analysis of Il22^TdT expression in NKp46⁺ and CCR6⁺ ILC3s (CD45⁺ CD3⁻ CD5⁻ CD90⁺ CD127⁺ cells) from Ncr1^GFPIl22^TdT mice from one of two independent experiments. d, Representative flow cytometry analysis of small intestine ILC3 subsets (pre-gated on CD45⁺ CD3⁻ CD5⁻ NK1.1⁻ CD127⁺ RORγt⁺ cells) from PBS or flagellin (Flag)-treated wild-type mice from one of five experiments. Absolute numbers of total, Il22^TdT+ and IL-22⁺ ILC3s (n = 9). Each bar corresponds to the mean ± s.e.m of the values obtained (*P < 0.05; **P < 0.01; ***P < 0.001; two-tailed Mann-Whitney test; exact P values are provided in the source data). e, Representative images of observable cells in Ncr1^GFPIl22^TdT mice by intravital imaging rom one of three independent experiments. f, Representative images and absolute numbers of Ncr1^GFP+Il22^TdT+ ILC3s in intestinal villi of from PBS or Flag-treated Ncr1^GFPIl22^TdT mice from one of three independent experiments (scale bar, 30 µm). g, Sample tracks of Ncr1^GFP+Il22^TdT+ ILC3s patrolling behaviors used in Fig. 2g.

Source data