Extended Data Fig. 3. Cancer stem cell (CSC) enrichment through nucleic acid transfer.
(a) Flow cytometry analysis of CSC surface markers in ‘receiving’ viable AT3 breast carcinoma and CT26 colon murine carcinoma cells upon coculturing with ‘donor’ cells of the same type previously treated with oxaliplatin (OXP; 300 µM, 48 h) alone or in combination with benzonase (BNZase; 200 IU ml−1, 48 h). Data are presented as mean ± s.e.m. and individual data points. Number of biologically independent experiments are reported. (b) Schematic representation of the extracellular vesicle (EV)-‘receiving’ cell coculture experimental protocol. (a) Ordinary one-way ANOVA test followed by Bonferroni’s correction.