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. 2022 Aug 24;23(9):1379–1392. doi: 10.1038/s41590-022-01290-3

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© The Author(s) 2022, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a,b) Multiparametric flow cytometry analysis of cancer stem cell (CSC) surface markers (a) and qRT–PCR analyses of the reported reprogramming factors (b) in the indicated parental (PAR) cells and the same cell lines engineered to overexpress or down-express KDM1B (Kdm1b^OVER and Kdm1b^KD). The histograms in (a) represent the percentage (mean ± s.e.m. and individual data points, n = 3 biologically independent experiments) of the indicated CSC subpopulation including CD133⁺CD24⁺CD44^+high (CD44H) MCA205 cells. qRT–PCR data are reported as mean fold change (FC) over untreated condition after intrasample normalization to Ppia expression levels. *P < 0.05, **P < 0.01, ***P < 0.001; the exact P values are in Supplementary Table 2. (c-e) Evaluation of the assessment of migration ability by transwell assay (c), therapeutic response to the reported immunogenic cell death (ICD) inducers and non inducers (d) and in vitro tumorigenicity and self-renewal potential by ELDA assay (e) in the indicated Kdm1b^OVER and Kdm1b^KD cells. Number of biologically independent samples (mean ± s.e.m. and individual data points for c and d) collected over three independent experiments is reported. (a,b) Ordinary one-way ANOVA test followed by Bonferroni’s correction as compared to control condition. (c-e) Unpaired two-sided Student’s t-test followed by Welch’s correction and two-tailed Mann–Whitney test. Exact calculations for ELDA assay are in Supplementary Table 2.

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