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. 2022 Jul 21;609(7927):622–629. doi: 10.1038/s41586-022-05116-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Schematic showing the components required for IL-25 signalling: IL-17RB, IL-25 and IL-17RA. b, Gel filtration chromatogram and SDS–PAGE gel image for the IL-25–IL-17RB complex (RB–IL-25). The highlighted area under the size-exclusion chromatography curve represents the pooled fractions that were concentrated and vitrified for cryo-EM. MW, molecular weight. c, Two-dimensional classifications of binary (2:2) IL-25–IL-17RB homodimeric complexes and higher-order IL-25–IL-17RB (6:6) complexes. Scale bars, 55 Å (2:2 2D class averages) and 125 Å (6:6 2D class averages). d, Cryo-EM map of the 2:2 IL-25–IL-17RB complex. e, Model of the 2:2 IL-25–IL-17RB complex. Dashed lines indicate unmodelled linkers to the transmembrane regions of the receptors. f, Cryo-EM map of the higher-order IL-25–IL-17RB (6:6) complex, comprising a trimer of IL-25–IL-17RB homodimers.