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. 2022 Jul 21;609(7927):622–629. doi: 10.1038/s41586-022-05116-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Workflow for cryo-EM data processing. Representative micrographs, reference free 2D averages, and cryo-EM maps at the various stages of processing. b, Local resolution estimation of the finalized cryo-EM map⁴². c, FSC curve of the complex reconstruction using gold-standard refinement calculated from unmasked and masked half maps. d, Orientational distribution of the complex reconstruction. e, Map vs model FSC for the 2:2 IL-25–IL-17RB binary complex.