Fig. 1.
IL-27 p28 deficiency aggravates aGVHD in mice. a–c BALB/c DCs were cocultured with CFSE-labeled T cells (ratio 1:10) from CD11c-p28f/f mice or control littermates, respectively. Proliferations were assessed by a 3H-TdR or b, c flow cytometry 5 days post coculture. d–f BALB/c recipients were transplanted with 1 × 107 WT BMs and 5 × 106 splenocytes from either WT or CD11c-p28f/f mice (n = 10–14 per group). Overall survival curve is depicted (d). Representative H&E stained sections and histological scores of aGVHD tissues from recipients 14 days post-transplantation are shown (e, f). g C57BL/6 or CD11c-p28f/f recipients were lethal irradiation and received 1 × 107 BMs and 7.5 × 107 splenocytes from BALB/c mice (n = 15 per group). The overall survival curve is depicted. Data are representative of three independent experiments and presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001