Fig. 4.
IL-27 p28 deficiency impairs Treg cell generation and promotes pathogenic IL-1R2+TIGIT+ CD4+T cells in the thymus. a Reclustering of T cell subpopulations in WT and CD11c-p28f/f mice. b Violin plots of the relative expressions of CD3D, CD4, and CD8. c Percent of different thymic T cell subsets determined by scRNA-seq analysis. d Populations and numbers of DP T cells in the thymus from WT and CD11c-p28f/f mice by flow cytometry. e t-SNE visualization of CD4 SP T cells. f Components of subclusters in CD4 SP cells. g Expression profile of genes involved in Treg development. h Volcano plot showing differential gene expression between Cluster 1 and Cluster 0 cells from CD11c-p28f/f and WT mice. i Violin plots showing the expression profile of T cell effector genes. Expression is measured as the log2-fold change. j GSEA of the upregulated gene set in Cluster 1 versus Cluster 0 in CD4 SP cells from CD11c-p28f/f relative to WT mice. k, l Populations of donor-derived IL-1R2+TIGIT+CD4+T cells were detected by FACS 7 days post-transplantation (n = 9–10 per group). m, n Percentages of IL-1R2+TIGIT+CD4+T cells in PBMCs were detected by FACS 30 days post-allo-HSCT. *P < 0.05; **P < 0.01; ***P < 0.001