Table 1.
Oligonucleotide sequences of primers used in the present study.
| Target organism (genetic marker) | Primer sequences (5′→3′) | Product size | Reference | |
|---|---|---|---|---|
| Mycoplasma spp. (16 S rRNA) | HBT-F: ATA CGG CCC ATA TTC CTA CG | 600 bp | Criado-Fornelio et al. (2003) | |
| HBT-R: TGC TCC ACC ACT TGT TCA | ||||
| Mycoplasma spp. (16 S rRNA) | UNI_16 S_mycF: GGC CCA TAT TCC TAC GGG AAG CAG CAG T | 1000 bp | Volokhov et al. (2011) | |
| UNI_16 S_mycR: TAG TTT GAC GGG CGG TGT ACA AGA CCT G | ||||
| Hepatozoon spp. (18 S rRNA) | H14Hepa18SFw: GAA ATA ACA ATA CAA GGC AGT TAA AAT GCT | 620 bp | Hodžić et al. (2015) | |
| H14Hepa18SRv: GTG CTG AAG GAG TCG TTT ATA AAG A | ||||
| Piroplasmida (18 S rRNA) | BTH-1F: CCT GAG AAA CGG CTA CCA CAT CT | 700 bp | Zintl et al. (2011) | |
| BTH-1R: TTG CGA CCA TAC TCC CCC CA | ||||
| GF2: GTC TTG TAA TTG GAA TGA TGG | 561 bp | |||
| GR2: CCA AAG ACT TTG ATT TCT CTC | ||||
| Cytauxzoon spp. (18 S rRNA) | 7549 F: GTC AGG ATC CTG GGT TGA TCC TGC CAG | 1726 bp | Millán et al. (2007) | |
| 7548 R: GAC TGA ATT CGA CTT CTC CTT CCT TTA AG | ||||
| Cyt-SSU-F2: CAT GGA TAA CCG TGC TAA TTG | 1335 bp | Panait et al. (2021b) | ||
| Cyt-SSU-R4: AGG ATG AAC TCG ATG AAT GCA | ||||
| Cytauxzoon spp. (CytB) | Cytaux_cytb_F1: CTT AAC CCA ACT CAC GTA CC | 1434 bp | Schreeg et al. (2013) | |
| Cytaux_cytb_R3: GGT TAA TCT TTC CTA TTC CTT ACG | ||||
| Cytaux_cytb_Finn: ACC TAC TAA ACC TTA TTC AAG CRT T | 1333 bp | Panait et al. (2021b) | ||
| Cytaux_cytb_Rinn: AGA CTC TTA GAT GYA AAC TTC CC | ||||
| Bartonella spp. (16 S–23 S rRNA) | bartgd_for: GAT GAT GAT CCC AAG CCT TC | 179 bp | Jensen et al. (2000) | |
| B1623_rev: AAC CAA CTG AGC TAC AAG CC | ||||
| Bartonella spp. (gltA) | BhCS.781p: GGG GAC CAG CTC ATG GTG G | 379 bp | Norman et al. (1995) | |
| BhCS.1137n: AT GCA AAA AGA ACA GTA AAC A | ||||
| Rickettsia spp. (23 S–5S rRNA) | ITS-F: GAT AGG TCG GGT GTG GAA G | 350-550 bp | Vitorino et al. (2003) | |
| ITS-R: TCG GGA TGG GAT CGT GTG | ||||
| Anaplasmataceae (16 S rRNA) | EHR16SD_for: GGT ACC YAC AGA AGA AGT CC | 345 bp | Parola et al. (2000) | |
| EHR16SR_rev: TAG CAC TCA TCG TTT ACA GC | ||||
| Filarioidea (COI) | H14FilaCOIFw: GCC TAT TTT GAT TGG TGG TTT TGG | 724 bp | Hodžić et al. (2015) | |
| H14FilaCOIRv: AGC AAT AAT CAT AGT AGC AGC ACT AA | ||||
Note: Supplementary data associated with this article.