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. 2022 Sep 15;5:969. doi: 10.1038/s42003-022-03871-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic of the cardiomyocyte-aperture interface with corresponding external (blue) and internal (orange) solutions. The substitution of which allows for multi-current acquisition from a single cell. b Shares of total CM numbers that showed successful measurements of any of the three parameters of interest (L-type calcium current (I_Ca,L), action potential (AP) duration at 90% repolarization (APD₉₀) and basal inward rectifier current (I_K1). The central total indicates the successful cohort of atrial and ventricular (Vent.) CMs in which all three parameters were successfully acquired. c A detailed overview of the CAPER protocol including sequential internal and/or external solution exchange during a single experimental run. The acetylcholine-activated inward rectifier current (I_K,ACh) is an optional addition to the protocol prior to the inward rectifier block with BaCl₂. d Three-dimensional visualization of I_Ca,L, APD₉₀, and I_K1 relationships in atrial (left) and ventricular (right) CMs. APD₉₀ is expressed as a shadow spectrum where darker colors indicate longer AP duration. e Time course of membrane capacitance (upper) and series resistance (R_series; lower) from a single plate (n = 5 CMs) over a full CAPER run. f Direct acquisition software screenshots from a single plate (single animal) showing three complete electrophysiological measurements from single cells. Y-axis indicates the membrane current expressed as picoamperes (p) or nanoamperes (n) in the screenshots of I_Ca,L (left) and I_K1 (right)_, or membrane voltage expressed as millivolts (m; center).