Fig. 2. Solution properties of nMazE6.

a Assigned 2D ¹H-¹⁵N HSQC spectrum of nMazE6. Sequence specific assignments are indicated on the spectrum. Horizontal dotted lines connect resonances of side chain amide protons. b Strip-plots from ¹⁵N-edited NOESY data showing sequential intra-subunit and cross-strand long-range NOEs observed across the dimer interface. NOEs are colored black (intra-residue), red (intra-subunit _i−1H^α- _iH^N), cyan (inter-subunit H^α-H^N), pink (inter-subunit H^N-H^N) and green (inter-subunit side chain-H^N). β-sheet topology constructed from the pattern of assigned NOEs is shown on the side. NOEs are indicated by colored broken lines. Proposed hydrogen bonding between donor-acceptor pairs in nMazE6 are indicated by black dotted lines.