Table 2.

Thermodynamic and kinetic parameters derived for interaction of nMazE6 with operator DNA constructs.

Thermodynamic parameters derived from ITC experiments for interaction of nMazE6 with different DNA
S. No.	Experiment	Kd (μM)	ΔG (kcal/mol)	ΔH (kcal/mol)	TΔS (kcal/mol)	n
1.	nMazE6-mazEF6 operator DNA	0.29 ± 0.04	−9.08 ± 0.22	−6.13 ± 0.15	2.95	0.93 ± 0.01
2.	nMazE6-mazEF6 operator DNA	0.22 ± 0.02	−9.24 ± 0.11	−6.49 ± 0.08	2.76	0.94 ± 0.01
3.	nMazE6- mazEF6 mutated DNA	–	–	–	–	–
4.	nMazE6-scramble DNA	–	–	–	–	–
5.	nMazE6-mazEF9 operator DNA	–	–	–	–	–

Thermodynamic and kinetic parameters derived from NMR titration experiments
	Experiment		Kd (μM)	kon (M−1 s−1)	koff (sec−1)
1.	nMazE6-mazEF6 operator DNA		0.09 ± 0.01	(147 ± 22) × 107	125 ± 4.04
2.	nMazE6-mazEF6 mutated DNA		8.59 ± 0.31	(17.30 ± 0.93) × 107	1486.44 ± 27.70
3.	nMazE6-mazEF9 operator DNA		20.60 ± 1.81	(8.2 ± 0.94) × 107	1696 ± 47.14