FIG. 3.

(A) Map of chromosomally encoded benzoate degradation genes from P. putida. (B) Map of the benA promoter region, diagram of the region incorporated to construct the benA-lacZ fusion plasmid, pCCH101, and nucleotide sequence of the benA promoter region. The putative BenR binding sites are heavily underlined. Introduced restriction sites are lightly underlined. The transcriptional and translational start sites are in bold. (C) Determination of the 5′ end of the benA transcript by primer extension. RNA was isolated from glucose-grown cells (lane 1) and glucose-benzoate-grown cells (lane 2) as described in the text. A sequence ladder was generated with the same primer (lanes C, T, A, and G). The first nucleotide in the transcript is shown in bold.