FIG. 6.
Effect of alterations in the CRP binding site in the clyA promoter region on the chromosome to match the consensus sequence (5′-AAATGTGATCTAGATCACATTT-3′). (Upper panel) MC4100 and MWK11 on a blood agar plate after incubation at 37°C for 17 h. (Lower panel) Detection of ClyA protein content in MC4100 and MWK11 by Western immunoblotting using a ClyA-specific antiserum (see Materials and Methods). Strains were grown in LB broth to late logarithmic phase. Approximately 107 bacteria were used for the extract loaded in each lane.