TABLE 2.

Plasmids used in this work

Plasmid	Relevant characteristics	Reference or source
pCH257	Suicide vector, Cmr	17
pFZY1	Mini-F transcriptional lacZ fusion vector, Cbr	28
pKO3	Gene replacement vector, Cmr	31
pLG339	Cloning vector, Tcr Kmr	54
pSL1180	Cloning vector, Cbr	7
pYMZ62	3.5-kb subclone of the clyA locus in pUC18	This laboratory
pYMZ80	1.6-kb subclone of the clyA locus in pUC18	This laboratory
pYMZ81	Same as plasmid pYMZ80, but with the 1.6-kb clyA locus in the opposite orientation	This laboratory
pYMZ83	Transcriptional clyA::lacZ fusion in pFZY1	This work
pMWK4	clyA::luxAB in pCH257	This work
pMWK9	pYMZ81 with altered CRP binding site: 5′-TTGTTTGATATAGATCACATTT-3	This work
pMWK10	pYMZ81 with altered CRP binding site: 5′-AAATGTGATCTAGATCACATTT-3′	This work
pMWK24	pYMZ81 with altered CRP binding site: 5′-TTGTGTGATATTTATCATATTA-3′	This work
pMWK28	pYMZ81 with altered CRP binding site: 5′-TTGTTTAATATTTATCATATTA-3′	This work
pMWK29	pYMZ81 with altered CRP binding site: 5′-TTGTCTAATATTTATCATATTA-3′	This work
pMWK31	pYMZ81 with altered clyA −10 sequence: TATGAAT→CACGAAC	This work
pMWK32	Subclone of the clyA locus from position −349 to −50 in pGEM-T Easy	This work
pMWK33	Subclone of the clyA locus from position −250 to +50 in pGEM-T Easy	This work
pMWK34	Subclone of the clyA locus from position −150 to +150 in pGEM-T Easy	This work
pMWK35	Subclone of the clyA locus from position −50 to +250 in pGEM-T Easy	This work
pMWK36	Subclone of the clyA locus from position +50 to +349 in pGEM-T Easy	This work
pMWK37	Subclone of the clyA locus from position +150 to +449 in pGEM-T Easy	This work
pMWK45	pJON78 with altered CRP binding site: 5′-AAATGTGATCTAGATCACATTT-3′	This work
pJON22	2.1-kb subclone of the slyAEC locus in pACYC177	This laboratory
pJON78	3.5-kb subclone of the clyA locus in pKO3	This laboratory
pMOJ2	slyA in-frame deletion in pKO3	This work
pDW300	crp gene in pLG339, formerly known as pLG339/CRP	2, 60
pGS24	fnr gene in pBR322	50
pGS215	FNR-V208R;S212G;G216K	53
pGS297	FNR-V208R;E209D	52