TABLE 3.
Expression of ClyA as evidenced by lysisa of erythrocytes in agar
| E. coli strain | Relevant characteristic(s) | ClyA expression under the following conditions
|
|
|---|---|---|---|
| Aerobic | Anaerobic | ||
| BSN26 | Wild type | − | − |
| BSN27 | hns | ++ | ++ |
| MWK6 | hns slyA | ++ | ++ |
| JON31 | hns | ++ | ++ |
| JON32 | hns crp | (+) | (+) |
| BEU701 | hns fnr | ++ | ++ |
| BEU705 | hns crp fnr | (+) | (+) |
| JON32/pDW300 | hns crp/p(CRP) | ++ | ++ |
| JON32/pLG339 | hns crp | (+) | (+) |
| BEU701/pDW300 | hns fnr/p(CRP) | ++ | ++ |
| BEU701/pLG339 | hns fnr | ++ | ++ |
| BEU701/pGS24 | hns fnr/p(FNR) | ++ | ++ |
| BEU701/pGS215 | hns fnr/p(FNR-V208R;S212G;G216K) | ++ | ++ |
| BEU701/pGS297 | hns fnr/p(FNR-V208R;E209D) | ++ | ++ |
| BEU701/pBR322 | hns fnr | ++ | ++ |
| BEU705/pDW300 | hns crp fnr/p(CRP) | ++ | ++ |
| BEU705/pLG339 | hns crp fnr | (+) | (+) |
| BEU705/pGS24 | hns crp fnr/p(FNR) | (+) | + |
| BEU705/pGS215 | hns crp fnr/p(FNR-V208R;S212G;G216K) | (+) | + |
| BEU705/pGS297 | hns crp fnr/p(FNR-V208R;E209D) | (+) | + |
| BEU705/pBR322 | hns crp fnr | (+) | (+) |
| M182/pJON22 | Wild type/p(SlyA) | ++ | ++ |
| M182/pACYC177 | Wild type | − | − |
| M182 crp/pJON22 | crp/p(SlyA) | (+) | (+) |
| M182 crp/pACYC177 | crp | − | − |
| JON31/pJON22 | hns/p(SlyA) | ++ | ++ |
| JON31/pACYC177 | hns | ++ | ++ |
| MWK6/pJON22 | hns slyA/p(SlyA) | ++ | ++ |
| MWK6/pACYC177 | hns slyA | ++ | ++ |
a
Lysis of erythrocytes was scored on blood agar plates as ++, lysis around individual colonies; +, lysis beyond the edge of the bacterial cell mass only; (+), weak lysis in the center of the bacterial cell mass only; or −, no lysis.