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. 2022 Jul 9;16(10):2373–2387. doi: 10.1038/s41396-022-01281-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Maximum-likelihood tree of a concatenated set of 122 archaeal-specific marker genes inferred with IQTREE (LG + C60 + F + G and 1000 ultrafast bootstrapping), rooted with the DPANN superphylum, showing the placement of the “Ca. Methylarchaeales” (in cyan) relative to 321 archaeal genomes. Ultrafast bootstrap (BS) value ≥95 are represented by black circles. Representative mcr-containing archaeal lineages available in public databases are included and expanded in the tree. The lineages assigned to the Euryarchaeota (recently reclassified as a superphylum consisting of three separate phyla: “Ca. Halobacteriota”, Methanobacteriota, and “Ca. Thermoplasmatota”) are classified at the order level. b The phylogenetic distribution of key methane metabolism related genes. For mtrA-H, fpoA-N, fpo-like, and atpA-K, they were regarded as present if ≥80% of the subunit genes constituting these complexes were identified. For other complexes, they were regarded as present only when all subunit genes for these complexes were found.