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. 2022 Jul 9;16(10):2373–2387. doi: 10.1038/s41396-022-01281-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Gene composition of contigs/scaffolds containing the gene cluster of heterodisulfide reductase (HdrDE) complex. Genes related to methane metabolism are highlighted with red, blue, yellow, and cyan. The hdrDE complex gene cluster is always adjacent to mcrABDG operon. b Gene composition of methanophenazine-reducing hydrogenase (VhtAGC) complex. Genes for VhtAGC were collocated on the same contig/scaffold, forming a transcriptional unit. c Structural model of b-type cytochromes in HdrDE and VhtAGC complexes showing the proposed heme ligation.