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. 2022 Sep 15;13:5409. doi: 10.1038/s41467-022-33152-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Measured values obtained from the echocardiogram in α-myosin heavy chain promoter-driven Cre (αMHC-Cre) PAC, and albumin promoter-driven Cre (Alb-Cre) PAC, C3 floxed αMHC-Cre (C3^fl/fl αMHC-Cre) PAC, and C3 floxed Alb-Cre (C3^fl/fl Alb-Cre) PAC mice (n = 3, a p = 0.0002, p < 0.0001, b p = 0.0015, p = 0.0147). The right ventricle (RV) contractile function (right ventricular fractional shortening [RV FS]) and RV size (right ventricular end-diastolic diameter [RVDd]) were evaluated. Data are presented as mean ± standard deviation (SD). c Representative images of Azan staining of the heart in C3^fl/fl αMHC-Cre PAC mice (n = 3). d Representative images of Azan staining of the heart in C3^fl/fl Alb-Cre PAC mice. LV, left ventricle (n = 3). e, f Quantified fibrotic area of the RV and LV in C3^fl/fl αMHC-Cre and C3^fl/fl Alb-Cre PAC model mice (n = 3, p = 0.0005, p = 0.039). Data are presented as mean ± SD. g–j qRT-PCR analysis of the expression of heart failure markers (Nppb and Myh7) and fibrotic markers (Col3a1 and Ccn2) in the LV, ventricular septum, and RV of αMHC-Cre PAC, and Alb-Cre PAC, C3^fl/fl αMHC-Cre PAC, and C3^fl/fl Alb-Cre PAC mice (n = 3, g p < 0.0001, p = 0.0003, h p < 0.0001, p < 0.0001, i p < 0.0001, p < 0.0001, j p < 0.0001, p = 0.0012). Data are presented as mean ± standard error of the mean. In qRT-PCR analysis, expression of target genes was normalised to that of Gapdh. Significance was assessed using a two-tailed unpaired Student’s t-test. *p < 0.05; **p < 0.01.