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. 2022 Sep 2;13:950345. doi: 10.3389/fendo.2022.950345

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Copyright © 2022 Li, Gao, Yuan, Wang, Xu, Wang, Liu, Guan, Zhang and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of migration, invasion, and colony formation in OVCAR3, SKOV3, and A2780 cells. (A) Detection of cell migration and invasion after CROT-overexpressing plasmid (oe-CROT) transfection for 48 h. (B) Detection of cell migration and invasion after CROT-siRNA (si-CROT) transfection for 48 h. (C) Detection of colony formation after CROT-overexpressing plasmid (oe-CROT) transfection for 2 weeks. (D) Detection of colony formation after CROT-siRNA (si-CROT) transfection for 2 weeks. Histograms show the statistical analyses. Assays were repeated at least three times. Data presented as mean ± SEM. *P <0.05; **P < 0.01; ***P < 0.001; ****P < 0.001; oe, overexpression of CROT; si, CROT-siRNA; CTL, negative control.