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. 2022 Sep 2;13:950345. doi: 10.3389/fendo.2022.950345

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Copyright © 2022 Li, Gao, Yuan, Wang, Xu, Wang, Liu, Guan, Zhang and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurement of apoptotic cells by flow cytometry. (A) Detection of apoptotic cells after CROT-overexpressing plasmid (oe-CROT) transfection in OVCAR-3, SK-OV-3, and A2780 for 48 h. (B) Detection of apoptotic cells after CROT-siRNA (si-CROT) transfection in OVCAR-3, SK-OV-3, and A2780 for 48 h. (C) Chemoresistant A2780-PTX cells were transfected with oe-CROT for 48 h, followed by 3 μM PTX treatment for 48 h. (D) The histogram shows the statistical analyses of (C) Assays were repeated at least three times. Data presented as mean ± SEM. *P <0.05; **P < 0.01; ***P < 0.001; ****P < 0.001; PTX, paclitaxel.