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. 2022 Sep 2;13:909207. doi: 10.3389/fendo.2022.909207

Figure 2.

Figure 2

Effects of Cx43 knockdown on HG-induced angiogenesis (A) Cx43 knockdown in hRECs was achieved through the transfection of the vector containing small hairpin RNA targeting Cx43 (sh-Cx43#1/2/3); Cx43 knockdown was confirmed using Immunoblotting. hRECs were subsequently transfected with sh-Cx43#2 (with better knocking down efficiency), cultured in normal or 60 mmol/L HG medium, and examined for tubule formation on Matrigel (B); ROS release using flow cytometry (C); the content of TNF-α, IL-1β, VEGFA, and ICAM-1 in culture medium using ELISA kits (D). n = 3, *p < 0.05, **p < 0.01 compared with normal+sh-NC group; ##p < 0.01 compared with HG+sh-NC group.