Table 1.
The sequence of RT-PCR primer and shRNA.
| RT-PCR primer (mouse) | Forward 5’-3’ | Reverse 5’-3’ |
|---|---|---|
| CD31 | ACCGGGTGCTGTTCTATAAGG | TCACCTCGTACTCAATCGTGG |
| TNF-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| IL-1β | CTGTGACTCATGGGATGATGATG | CGGAGCCTGTAGTGCAGTTG |
| VEGFA | CGATGAAGCCCTGGAGTGCGT | ACAAACAAATGCTTTCTCCGCTCTG |
| ICAM-1 | GGCATTGTTCTCTAATGTCTCCG | TGTCGAGCTTTGGGATGGTAG |
| Tubulin | GCATTAACTACCAGCCTCCCAC | CGCCTTCCACAGAATCCACAC |
| Vector construction primer (human) | Forward 5’-3’ | Reverse 5’-3’ |
| Cx43 overexpression | CTACCGGACTCAGATCTCGAGATGGGTGACTGGAGCGCC | GTACCGTCGACTGCAGAATTCCTAGATCTCCAGGTCATCAGGCC |
| Sh-NC | GATCCGCCAAGCCCTTGTCTTCACAACTCGAGTTGTGAAGACAAGGGCTTGGCTTTTTG | AATTCAAAAAGCCAAGCCCTTGTCTTCACAACTCGAGTTGTGAAGACAAGGGCTTGGCG |
| Sh-Cx43 1 | GATCCGTATTGAAGAGCATGGTAAGGCTCGAGCCTTACCATGCTCTTCAATACTTTTTG | AATTCAAAAAGGATTGAAGAACACGGCAAGGCTCGAGCCTTACCATGCTCTTCAATACG |
| Sh-Cx43 2 | GATCCGGGTCCTGCAGATCATATTTGCTCGAGCAAATATGATCTGCAGGACCCTTTTTG | AATTCAAAAAGGGTCCTGCAGATCATATTTGCTCGAGCAAATATGATCTGCAGGACCCG |
| Sh-Cx43 3 | GATCCGCCACTAGCCATTGTGGACCACTCGAGTGGTCCACAATGGCTAGTGGCTTTTTG | AATTCAAAAAGCCACTAGCCATTGTGGACCACTCGAGTGGTCCACAATGGCTAGTGGCG |
| Vector construction primer (mouse) | Forward 5’-3’ | Reverse 5’-3’ |
| lv-sh-Cx43 | GATCCGGGTCCTTCAGATCATATTCGCTCGAGCGAATATGATCTGAAGGACCCTTTTTG | AATTCAAAAAGGGTCCTTCAGATCATATTCGCTCGAGCGAATATGATCTGAAGGACCCG |
| lv-Sh-NC | GATCCGCCAAGCCCTTGTCTTCACAACTCGAGTTGTGAAGACAAGGGCTTGGCTTTTTG | AATTCAAAAAGCCAAGCCCTTGTCTTCACAACTCGAGTTGTGAAGACAAGGGCTTGGCG |