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. 2022 Sep 2;13:925008. doi: 10.3389/fpls.2022.925008

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Copyright © 2022 Jugler, Grill, Eidenberger, Karr, Grys, Steinkellner, Lake and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis of purified P-4H2 IgM. Recombinant 4H2 IgM purified from Nicotiana benthamiana leaves was separated under reducing (A,B) or non-reducing (C) conditions by SDS-PAGE and proteins were then transferred to PVDF membranes. The membranes were incubated with antibodies against human kappa chain (A,C) or human mu chain (B) to verify protein identity and IgM assembly. Lane 1, Humanized P-4H2 IgM (1 μg per lane in A, 0.5 μg per lane in B,C), Lane 2, Human kappa IgM isotype control (1 μg per lane in A, 0.5 μg per lane in B,C).