Figure 5.

Plant-Made 4H2 Isotypes Specifically Bind to Target Antigen. Serial dilutions of P-4H2 IgG and P-4H2 IgM isotypes were incubated with CTS1 immobilized on ELISA plates. An HRP-conjugated anti-human IgG or anti-human IgM secondary antibody was used to detect the specific interaction between the plant-made IgG or IgM isotype (P-4H2 IgG, P-4H2 IgM) with CTS1, respectively. The parental mouse IgG (M-4H2 IgG) was used as a positive control, which was detected with an anti-mouse IgG secondary antibody conjugated to HRP. Mean ± SEM is plotted from three independent experiments.