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. 2022 Sep 2;10:926180. doi: 10.3389/fcell.2022.926180

FIGURE 2.

FIGURE 2

5-aza affects ASC morphology, actin cytoskeleton distribution and growth. (A) Phase-contrast photomicrographs showing the morphology of ASCs pretreated with 5-aza or DMSO. Representative areas of the full image are presented as enlargements in lower panels. Scale bars: 100 μm. (B) Fluorescence images showing phalloidin labeling of actin filaments (red) in ASCs pretreated with 5-aza or DMSO. Nuclei were visualized using 4′, 6-diamido-2-phenylindole dihydrochloride (DAPI) (blue). Representative areas of the full image are presented as enlargements in lower panels. Scale bars: 10 μm. (C) Fluorescence images showing Vinculin labeling of focal adhesions (green) in ASCs pretreated with 5-aza or DMSO. Representative areas of the full image are presented as enlargements in lower panels. Scale bars: 10 μm. (D) Growth kinetics of ASCs pretreated with 5-aza or DMSO. (E) Population doubling time calculated from the exponential growing phase of ASCs. Bars represent means ± SD of three independent experiments, each performed in triplicate.