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. 2022 Aug 17;298(9):102385. doi: 10.1016/j.jbc.2022.102385

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Analysis of substrates and products for the ASNS reaction in fibroblasts incubated in the presence or absence of Asn. Primary cultures of fibroblasts from the ASNSD family and a nonrelated WT fibroblast cell line were incubated in DMEM ± Asn for 24 h. Cell extracts (A) and the extracellular media (B) were collected for amino acid analysis by GC-MS. The results for Asn, Asp, Gln, and Glu are presented as “arbitrary units,” calculated as the averages of triplicate determinations ± SDs of the relative amount of each amino acid per 10⁶ cells. To better illustrate the changes in Asn, the ratios of the values for DMEM-Asn/DMEM+Asn were calculated and are shown as an insert. Where not shown, the SD marks are within the bar. An asterisk indicates that the p value was ≤ 0.05 compared to the value obtained for the WT cells. ASNS, asparagine synthetase.

Analysis of substrates and products for the ASNS reaction in fibroblasts incubated in the presence or absence of Asn. Primary cultures of fibroblasts from the ASNSD family and a nonrelated WT fibroblast cell line were incubated in DMEM ± Asn for 24 h. Cell extracts (A) and the extracellular media (B) were collected for amino acid analysis by GC-MS. The results for Asn, Asp, Gln, and Glu are presented as “arbitrary units,” calculated as the averages of triplicate determinations ± SDs of the relative amount of each amino acid per 10⁶ cells. To better illustrate the changes in Asn, the ratios of the values for DMEM-Asn/DMEM+Asn were calculated and are shown as an insert. Where not shown, the SD marks are within the bar. An asterisk indicates that the p value was ≤ 0.05 compared to the value obtained for the WT cells. ASNS, asparagine synthetase.