Intracellular localization of GnT-IVa and GnT-IVb. DKO cells transfected with a plasmid for expression of GnT-IVa or GnT-IVb were stained with anti-myc (green), antibodies for marker proteins (Golgin-97 for the Golgi, calnexin for the ER, or N-cadherin for the plasma membrane) (red), and DAPI (blue). The scale bar represents 10 μm. DAPI, 4′,6-diamidino-2-phenylindole; DKO, double KO; ER, endoplasmic reticulum; GnT, N-acetylglucosaminyltransferase.