Figure 3.

Loss of Idh3b disrupted cellular metabolism in the retina.A, schematic of TCA cycle metabolism. B, fold change of TCA cycle metabolites in retina from KO mice. N = 4, ∗p < 0.05. C, fold change of ¹³C-glucose-derived metabolites in the retina from KO mice. N ≥ 4, ∗p < 0.05. D–F, Western blot analysis of IDH1 and IDH2 in the retina from mice at P200. D, representative Western blot image. E and F, semiquantification of IDH1 and IDH2 level in retina. β-Tubulin (β-Tub) was used as loading control. N ≥ 3. G, enzymatic activity of IDH1 and IDH2 under different isocitrate concentrations in the retina from HET and KO mice. N ≥ 4. HET, heterozygous; IDH, isocitrate dehydrogenase; TCA, tricarboxylic acid.