Skip to main content
. 2022 Aug 17;298(9):102387. doi: 10.1016/j.jbc.2022.102387

Figure 6.

Figure 6

Acrosome biogenesis defects in Idh3b KO mice.A–J, representative images for immunofluorescent staining of acrosome and Golgi at different stages in testicular tubular sections from mice at P50. Red, GM130; blue, DAPI (4′,6-diamidino-2-phenylindole); and green, PNA. Arrowhead: defected acrosome. K–V, ultrastructure analysis of acrosome biogenesis at Golgi, cap, acrosome, and maturation stages. Blue arrows, Golgi; yellow arrows: acrosome (Ac), nucleus (Nu). W, table of proteins essential for acrosome biogenesis. ACRV1, acrosomal vesicle protein 1; GOPC, Golgi associated PDZ and coiled-coil motif; Hsp90B1, heat shock protein 90 beta family member 1; PICK1, protein interacting with PRKCA 1. X, Western blot analysis of listed proteins in testis from HET and KO mice at P50. HET, heterozygous.