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. 2022 Sep 2;13:912797. doi: 10.3389/fphys.2022.912797

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Copyright © 2022 Choi, Yadav, Wang, Lorentz, Lourenco, Callaway and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total antioxidant capacity, glutathione (GSH) and oxidized glutathione (GSSG) concentrations, and activities of superoxide dismutase (SOD) in broiler chickens fed different concentrations (0, 0.5, 1.5, and 2.5 g/kg) of tannic acid on D 21. The treatment groups were compared using one-way ANOVA followed by the Tukey’s multiple comparison. Different superscripts differ at p < 0.05. Orthogonal polynomial contrasts analysis was conducted to see linear pattern (L) and quadratic pattern (Q) among the treatments. To calculate the optimal dosage of tannic acid in broilers, segmented linear regression was conducted, and X0 (inflection point) with p values for slope 1 (lower than X0) and slope 2 (higher than X0) were presented. Reduced GSH = Total GSH – 2 × GSSG.