TABLE 1.
Ingredients and nutrient compositions of basal diets (As-fed basis).
| Items | D 0 to 14 | D 14 to 21 |
|---|---|---|
| Ingredients | ||
| Corn | 650.97 | 683.74 |
| Soybean meal (480 g crude protein/kg) | 295.05 | 260.05 |
| Defluorinated Phosphate | 16.62 | 14.96 |
| Filler 1 | 10 | 10 |
| Soybean oil | 8.27 | 12.91 |
| limestone | 6.45 | 6.24 |
| DL-Methionine 99% | 3.18 | 2.94 |
| L-Lysine HCl 78% | 3.01 | 2.85 |
| Vitamin Premix 2 | 2.5 | 2.5 |
| Common Salt | 1.45 | 1.66 |
| L-threonine | 1.20 | 0.86 |
| Mineral Premix 3 | 0.8 | 0.8 |
| Monensin sodium 4 | 0.5 | 0.5 |
| Total | 1,000 | 1,000 |
| Calculated energy and nutrient value, % | ||
| Metabolizable energy, Kcal/kg | 3,000 | 3,060 |
| Crude protein | 20.59 | 19.13 |
| SID 5 Methionine | 0.61 | 0.57 |
| SID Total sulfur amino acids | 0.88 | 0.82 |
| SID Lysine | 1.17 | 1.07 |
| SID Threonine | 0.78 | 0.7 |
| Total calcium | 0.87 | 0.8 |
| Available phosphate | 0.44 | 0.4 |
Sand and tannic acid were added to obtain desired tannic acid dosages in the feed as follows: Tannic acid 0 (TA0): sand 10 g/kg + tannic acid 0 g/kg; Tannic acid 0.5 (TA0.5): sand 9.5 g/kg + tannic acid 0.5 g/kg; Tannic acid 1.5 (TA1.5): sand 8.5 g/kg + tannic acid 1.5 g/kg; Tannic acid 2.5 (TA2.5): sand 7.5 g/kg + tannic acid 2.5 g/kg; Tannic acid was purchased from Sigma–Aldrich (St Louis, MO, United States).
Vitamin mix provided the following in mg/100 g diet: thiamine-HCl, 1.5; riboflavin, 1.5; nicotinic acid amide, 15; folic acid, 7.5; pyridoxine-HCl, 1.2; D-biotin 3, 1.5; vitamin B-12 (source concentrations, 0.1%), 2; choline-Cl, 3; D-calcium pantothenate, 4; menadione sodium bisulfite, 1.98; α-tocopherol acetate (source 500,000 IU/g), 22.8; cholecalciferol (source 5,000,000 IU/g), 0.09; retinyl palmitate (source 500,000 IU/g), 2.8; ethoxyquin, 13.34; I-inositol, 2.5; and dextrose, 762.2.
Mineral mix provided the following in g/100 g diet: Ca(H2PO4)2 · H2O, 3.62; CaCO3, 1.48; KH2PO4, 1.00; Na2SeO4, 0.0002; MnSO4 · H2O, 0.035; FeSO4 · 7H2O, 0.05; MgSO4 · 7H2O, 0.62; KIO3, 0.001; NaCl, 0.60; CuSO4 · 5H2O, 0.008; ZnCO3, 0.015; CoCl2 · 6H2O, 0.00032; NaMoO4 · 2H2O, 0.0011; KCl, 0.10; and dextrose, 0.40.
Coban 90, Elanco Animal Health, Greenfield, IN, United States
SID, tandardized ileal digestible.