FIGURE 3.

Effect of consecutive histamine applications on Ca²⁺ signal in WI-38 human lung fibroblasts WI-38. (A) Representative recording of a triple application of histamine (300 μM) in one cell, each followed by a washout with physiological saline solution (PSS). (B) Mean ± SE of peak and plateau amplitudes of the Ca²⁺ transient evoked by 3 consecutive histamine applications: first application (1st), second application (2nd), third application (3rd). (C) Mean ± SE of the number of oscillations measured over 400 s after each histamine application. The number in the figure represents the number of cells studied. Comparison between groups was performed using the Kruskal–Wallis test (* = p ≤ 0.05; ns = no statistically relevant differences between groups).