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. 2022 Sep 2;10:991659. doi: 10.3389/fcell.2022.991659

TABLE 1.

Primer sequences used for reverse transcription/polymerase chain reaction.

Gene Primer sequences Size (bp) Accession number
Orai1 Forward-5′-AGTTACTCCGAGGTGATGAG-3′ 257 NM_032790.3
Reverse-5′-ATGCAGGTGCTGATCATGAG-3′
Orai2 Forward-5′-CCATAAGGGCATGGATTACC-3′ 334 NM_001126340.1 variant 1
Reverse-5′-CAGGTTGTGGATGTTGCTCA-3′ NM_032831.2 variant 2
Orai3 Forward-5′-CCAAGCTCAAAGCTTCCAGCC-3′ 159 NM_152,288.2
Reverse-5′-CAAAGAGGTGCACAGCCACCA-3′
Stim1 Forward-5′-CCTCAGTATGAGGAGACCTT-3′ 347 NM_003156.3
Reverse-5′-TCCTGAAGGTCATGCAGACT-3′
Stim2 Forward-5′-AAACACAGCCATCTGCACAG-3′ 186 NM_020860.2
Reverse-5′-GGGAAGTGTCGTTCCTTTGA -3′
InsP3R1 Forward 5′-TCA​ACA​AAC​TGC​ACC​ACG​CT-3′ 180 ENSG00000150995
Reverse 5′-CTC​TCA​TGG​CAT​TCT​TCT​CC-3′
InsP3R2 Forward 5′-ACCTTGGG GTTAGTGGATGA-3′ 158 ENSG00000123104
Reverse 5′-CCT​TGT​TTG​GCT​TGC​TTT​GC-3′
InsP3R3 Forward 5′-TGG​CTT​CAT​CAG​CAC​TTT​GG-3′ 173 ENSG00000096433
Reverse 5′-TGT​CCT​GCT​TAG​TCT​GCT​TG-3′
RyR1 Hs00166991 Thermo Fisher Scientific 75 NM_000540.3
RyR2 Hs00181461 Thermo Fisher Scientific 65 NM_001035
RyR3 Hs00168821 Thermo Fisher Scientific 63 NM_001036.6
Trpc1 Forward 5′-ATC​CTA​CAC​TGG​TGG​CAG​AA-3′ 307
Reverse 5′-AAC​AAA​GCA​AAG​CAG​GTG​CC-3′
Trpc3 Forward 5′-GGA​GAT​CTG​GAA​TCA​GCA​GA-3′ 336 NM_001130698.1 v
Reverse 5′-AAG​CAG​ACC​CAG​GAA​GAT​GA-3′ NM_003305.2 variant
Trpc4 Forward 5’-ACC​TGG​GAC​CTC​TGC​AAA​TA-3′ 300 NM_016179.2 va
Reverse 5′-ACA​TGG​TGG​CAC​CAA​CAA​AC-3′ NM_001135955.1 va
NM_001135956.1 v
NM_001135957.1 va
NM_003306.1 va
NM_001135958.1 va
Trpc5 Forward 5′-GAG​ATG​ACC​ACA​GTG​AAG​AG-3′ 221 NM_012471.2
Reverse 5′-AGA​CAG​CAT​GGG​AAA​CAG​GA-3′
Trpc6 Forward 5′-AGC​TGT​TCC​AGG​GCC​ATA​AA-3′ 341 NM_004621.5
Reverse 5′-AAG​GAG​TTC​ATA​GCG​GAG​AC-3′
Trpc7 Forward 5′-CAC​TTG​TGG​AAC​CTG​CTA​GA-3′ 387 NM_020389.1
Reverse 5′-CAT​CCC​AAT​CAT​GAA​GGC​CA-3′