Fig. 2.

Intracellular uptake, cytotoxicity and ROS generation. A) CLSM images of cells treated with EPI, EHGZ, and mEHGZ after incubation of them with 4T1 cells for 2 h (blue: cell nuclei; red: EPI; scale bar = 20 μm). B) Flow cytometry analysis of the uptake efficiency of EPI, EHGZ and mEHGZ by 4T1 cells. Cytotoxicity of EPI, EZ, GHZ, EHGZ, and mEHGZ at different concentrations against 4T1 cells via the CCK-8 assay C), visualization by live and dead stained kit E) (Scale bar = 100 μm, green: live cells; blue: dead cells); Flow cytometry analysis of live and dead staining 4T1 cells via Annexin V-apoptotic assay kit F). Intracellular ROS generation after treatment with EPI, EZ, GHZ, EHGZ, and mEHGZ was detected Flow cytometry quantification D) and fluorescence microscope G). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.