Fig. 3.

In vitro ICD effect induced by the self-amplified nanosystem. A) Schematic diagram of a cascade process to generate cytotoxic ·OH and deplete intracellular GSH. B) GSH content in 4T1 cells after co-culture with EPI, EZ, GHZ, EHGZ, and mEHGZ for 12 h. C) ATP released in the supernatant after treatment with different preparations. D-E) CLSM images for HMGB1 distribution and CRT exposure in 4T1 cells after treatment of them with EPI, EZ, GHZ, EHGZ, and mEHGZ at an EPI concentration of 2 μg/mL for 4 h (blue: cell nuclei; red: HMGB1 or CRT; scale bar = 50 μm). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.