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. 2022 Sep 13;21:299–312. doi: 10.1016/j.bioactmat.2022.08.028

Fig. 4.

Fig. 4

DCs maturation and cytokines secretion induced by the cell membrane-coated biomimetic nanoparticles. A) Schematic illustration of co-culture of 4T1 cells and BMDCs after 4T1 cells were incubated with EPI, EZ, GHZ, EHGZ, and mEHGZ at an EPI concentration of 1 μg/mL for 12 h. B) The percentage of mature BMDCs (CD80+CD11c+) in the co-culture experiment measured by flow cytometry. C) The percentage of CD11C+CD80+CD86+ cells in the tumor tissue after treatment with EPI, EZ, GHZ, EHGZ, mEHGZ, anti-PD-L1 antibody, and mEHGZ + anti-PD-L1 antibody at an EPI dose of 3 mg/kg and 100 μg anti-PD-L1 antibody. D) CLSM images of CRT exposure on the tumor cell membrane in the tumor tissue after treatment with EPI, EZ, GHZ, EHGZ, mEHGZ, anti-PD-L1 antibody, and mEHGZ + anti-PD-L1 antibody three times. (blue: cell nuclei; red: CRT; scale bar = 100 μm). E-G) The concentration of IFN-γ, TNF-α, and IL-6 in the supernatant extracted from the tumor tissue after various treatments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.