Table 1.

List of primers used in quantitative PCR (qPCR).

Primer	Primer sequence (5’-3’)	Target Gene	Tm (°C)	Length	References
rpoA_F	GCAAATCTGGCATTGGCCCT	rpoA	61.6	195	(Pearson and Mobley, 2008)
rpoA_R	TAGGGCGCTCATCTTCTTCCG		61.9
umoC_F	CACAAGCCAGCAGTACTTCA	umoC	58.1	144	(Aygül et al., 2019)
umoC-R	GTGACTCTATCGCGGCTAAA		57.5
flhC_F	CGCACATCAGCCTGCAAGT	flhC	61.3	90	(Aygül et al., 2019)
flhC_R	GCAGGATTGGCGGAAAGTT		58.7
flhD_F	TGCCCGTTTCTTTGTAGCAGA	flhD	60	148	(Pearson and Mobley, 2008)
flhD_R	CCGGTTTGAAGACAGCGAAA		59
flhDC_F	CGCACATCAGCCTGCAAGT	flhDC	61.2	90	(Wang et al., 2008)
flhDC_R	GCAGGATTGGCGGAAAGTT		58
mrpA_F	ATTGCGGGCTCTGCATTA	mrpA	61	113	This study
mrpA_R	GGAATACGTTGAGCACCTGA		61
mrpJ_F	AACGTAAAGAGCTGGGTTAT	mrpJ	54.3	81	(Aygül et al., 2019)
mrpJ_R	GTTCATAGCGAGAAAACTGT		53.6
rcsB_F	GCGCTTATTTGCCGAAGGTTT	rcsB	60.4	107	(Pearson and Mobley, 2008)
rcsB_R	CACCGAGCTTCATCATGGCTG		61.6
rcsD_F	TCCGTATCGTACTTCCATACC	rcsD	56.4	86	(Aygül et al., 2019)
rcsD_R	GGCGAGTTTTGCAGTTATACC		57.6
speB_F	GCGGGATCAAGGCAATCAAT	speB	68.9	126	(Aygül et al., 2019)
speB_R	CTTTACTGTCTTGGATGCTGC		57.2
glnA-F	CGCCCAATGGTAAAAGGAGG	glnA	59	103	(Aygül et al., 2019)
glnA_R	CCACTAAGCCCATCTCTTCCA		59
luxS_F	ACGATGAAAACACCCTCTGG	luxS	57.8	125	This study
luxS_R	CGCATGAAACCCGCAAATAAG		58.8